Method of defining cell types by probing comprehensive expression libraries with amplified RNA
US6110711A · kind A · utility
24Cited by
2References
21Claims
0Family size
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Key dates
| Filing date | Dec 15, 1998 |
| Grant date | Aug 29, 2000 |
| Priority date | — |
| Expiry date | Dec 15, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6897
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention provides methods and compositions for defining a cell type, generally involving the steps of (a) amplifying the mRNA of a single cell of a heterogenous population of cells; (b) probing a comprehensive expression library with the amplified mRNA to define a gross expression profile of the cell; and (c) comparing the gross expression profile of the cell with a gross expression profile of one or more other cells to define a unique expression profile of the cell, wherein the unique expression profile of the cell provides a marker defining the cell type.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.