Expression plasmid, a fusion protein, a transfected eukaryotic cell line, a method of producing foreign proteins, a foreign protein preparation as well as a pharmaceutical composition

US6114146A · kind A · utility

Assignee

• Baxter Aktiengesellschaft · AT

Inventors

• Sabine E. Herlitschka · Salzburg, AT
• Uwe Schlokat · Orth an der Donau, AT
• Falko-Guenter Falkner · Orth an der Donau, AT
• Friedrich Dorner · Wien, AT

Key dates

• Filing date: Nov 14, 1995
• Grant date: Sep 5, 2000
• Expiry date: Nov 14, 2015

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2840/203
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The invention describes an expression plasmid containing a dicistronic transcription/translation unit, which unit comprises a sequence for a foreign protein and a sequence for a fusion protein, the fusion protein containing at least one selection marker and at least one amplification marker. Further described is a method of producing foreign proteins by using the plasmids according to the invention, as well as cell lines transformed with the plasmid according to the invention.