Immobilized proteins with specific binding capacities and their use in processes and products
US6114147A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Nov 17, 1997 |
| Grant date | Sep 5, 2000 |
| Priority date | — |
| Expiry date | Nov 17, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/75
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method is provided for immobilizing a binding protein capable of binding to a specific compound, using recombinant DNA techniques for producing said binding protein or a functional part thereof. The binding protein is immobilized by producing it as part of a chimeric protein also comprising an anchoring part derivable from the C-terminal part of an anchoring protein, thereby ensuring that the binding protein is localized in or at the exterior of the cell wall of the host cell. Suitable anchoring proteins are yeast .alpha.-agglutinin, FLO1 (a protein associated with the flocculation phenotype in S. cerevisiae), the Major Cell Wall Protein of lower eukaryotes, and a proteinase of lactic acid bacteria. For secretion the chimeric protein can comprises a signal peptide including those of .alpha.-mating factor of yeast, .alpha.-agglutinin of yeast, invertase of Saccharomyces, inulinase of Kluyveromyces, .alpha.-amylase of Bacillus, and proteinase of lactic acid bacteria. Also provided are recombinant polynucleotides encoding such chimeric protein, vectors comprising such polynucleotide, transformed microorganisms having such chimeric protein immobilized on their cell will, and a proces…
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