Production of proteins by cell culture
US6117652A · kind A · utility
1Cited by
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2Claims
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Inventor
Key dates
| Filing date | May 14, 1997 |
| Grant date | Sep 12, 2000 |
| Priority date | — |
| Expiry date | May 14, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2500/36
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods for enhancing the production of tissue plasminogen activator (tPA) in cell culture are disclosed. The methods involve culturing tPA-producing cells in growth media supplemented with an alkanoic acid or salt thereof at a concentration which enhances tPA production. The most preferred methods utilize butyric acid or sodium butyrate at a concentration of between 0.1 mM and 10 mM.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.