Manufacture and use of polypeptides tagged using binding molecules
US6117976A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 2, 1996 |
| Grant date | Sep 12, 2000 |
| Priority date | — |
| Expiry date | May 2, 2016 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/4727
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Molecules comprise (i) a polypeptide (such as calmodulin) which has calcium-dependent binding affinity for ligand and (ii) another polypeptide, the polypeptides preferably being joined by a peptide bond and produced by recombinant expression from a gene fusion. The molecules are useful in detection, immobilization, targeting and purification, cell-labelling, and band-shift assays for determining binding of a member of a specific binding pair (sbp) for complementary sbp member. For purposes of band-shift assays, polypeptide (i) need not have calcium-dependent binding affinity for a ligand, but should have a dissociation constant for a ligand of 10 nM or less, measured at a pH of between 6 and 9 at 20.degree. C. In an alternative embodiment, a calmodulin-binding polypeptide which is, or is derived from, mastoparan is joined, as polypeptide (i) instead of a binding polypeptide, to the other polypeptide.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.