Microfabricated device and method for multiplexed electrokinetic focusing of fluid streams and a transport cytometry method using same
US6120666A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jun 16, 1998 |
| Grant date | Sep 19, 2000 |
| Priority date | — |
| Expiry date | Jun 16, 2018 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T137/2082
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A microfabricated device and method for electrokinetic transport of a liquid phase biological or chemical material is described. In accordance with one aspect of the present invention there is provided a microchip that is adapted for the simultaneous spatial confinement of electrokinetically driven fluidic material streams on a substrate. The apparatus includes a focusing chamber formed in a surface of the substrate and in fluid communication with two sample fluid channels and three focusing fluid channels. The device further includes electromotive means operatively connected to the sources of the sample fluid and the source of focusing fluid for electrokinetically driving the respective streams of the sample and focusing fluids through the respective channels into the focusing chamber such that the focusing fluid streams spatially confine the first and second sample fluid streams within the focusing chamber. In accordance with another aspect of this invention, there is provided a cytometry method for analyzing microscopic particles in a fluid medium on a microchip by utilizing the focusing function of the microchip. In the disclosed cytometry process the width of the fluid stream …
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.