Immunochemical determination of multivalent analytes

US6120990A · kind A · utility

Assignee

• Dade Behring Marburg GmbH · DE

Inventors

• Stefan Brust · Marburg, DE
• Hans-Peter Hauser · Marburg, DE
• Stefan Knapp · Marburg, DE
• Helmut Peters · Marburg, DE

Key dates

• Filing date: Jun 4, 1998
• Grant date: Sep 19, 2000
• Expiry date: Jun 4, 2018

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/54306
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present invention is directed to an immunochemical method for qualitatively or quantitatively detecting an analyte in which a first and a second binding component are contacted with the analyte, followed by detection of the binding of the binding components to the analyte. According to the invention, both of the binding components are prepared recombinantly in the same host using different vectors (V1 and V2) for expressing fusion proteins, with the first binding component being expressed as a fusion protein F1 in vector V1 and the second binding component being expressed as a fusion protein F2 in vector V2. The novel method, which can also be employed for simultaneously determining several analytes, is more sensitive and specific than prior known methods.