Potent antigen presenting cell composition
US6121044A · kind A · utility
6Cited by
0References
7Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jul 12, 1995 |
| Grant date | Sep 19, 2000 |
| Priority date | — |
| Expiry date | Jul 12, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2740/16122
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A method of obtaining a blood-cell fraction enriched for potent antigen presenting cells is disclosed. The method includes obtaining a monocyte-depleted lymphocyte fraction, culturing the cell fraction in a serum-free medium for a period sufficient to produce a morphological change in dendritic-precursor cells to cells having the morphology of dendritic cells, harvesting non-adherent cells produced by said culturing, and enriching the portion of dendritic cells in the harvested cells by density centrifugation. Also disclosed is a PAP cell composition containing cells enriched for PAP activity in a collagen matrix.
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