Patent · US Expired

Device and method for magnetic separation of biological molecules

US6126835A · kind A · utility

42Cited by
3References
72Claims
0Family size

Assignee

Inventors

Key dates

Filing dateFeb 1, 1999
Grant dateOct 3, 2000
Priority date
Expiry dateFeb 1, 2019

Classification

  • Technology area (CPC Y)Emerging Cross-Sectional Technologies
  • CPC primaryY10S436/824
  • WIPO fieldChemical engineering
  • WIPO sectorChemistry

Abstract

Provided is a magnetic separation device comprising a container having one or more outer surfaces; at least one magnetic sheet; and a physical coupler that is used to detachably secure the container to the magnetic sheet. A method of using the magnetic separation device according to the present invention comprises obtaining a fluid containing a mixed population of biological molecules, from which it is desired to separate at least one subpopulation of biological molecules; mixing the fluid with a magnetic separation reagent; contacting the mixture with the fluid holding chamber of the magnetic separation device; incubating the mixture for a sufficient time to allow for complexes to form between the subpopulation of biological molecules and the magnetic separation reagent; positioning the magnetic separation device in a position that magnetically attracts the complexes towards the magnetic sheet, and thereby holds them in position in the container; and removing the remainder of the fluid from the magnetic separation device.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.