Use of an alcohol-salt buffer wash for the efficient recovery of mycobacteria and mycobacterial DNA from respiratory sediment
US6126839A · kind A · utility
1Cited by
0References
15Claims
0Family size
Inventors
Key dates
| Filing date | Jan 27, 1998 |
| Grant date | Oct 3, 2000 |
| Priority date | — |
| Expiry date | Jan 27, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12R2001/32
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to methods for concentrating bacteria from a viscous biological sample. The methods involve adding to the sample a water-soluble, density-lowering agent having a density of 0.7 to 0.9 g/ml and a boiling point greater than 50.degree. C. The invention also relates to methods for concentrating bacteria and free bacterial nucleic acids from a biological sample that involve mixing with the sample a density-lowering agent and a monovalent salt.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.