Purification cloning of peptides
US6127150A · kind A · utility
3Cited by
26References
20Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Sep 5, 1997 |
| Grant date | Oct 3, 2000 |
| Priority date | — |
| Expiry date | Sep 5, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/75
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention is directed to a protein purification construct having three tandem, coupled segments composed of a binding protein, an interconnecting linker and a variable fused polypeptide which incorporates the one or more copies of a product peptide. The binding protein is a mammalian or human carbonic anhydrase, or a modified version of the carbonic anhydrase. The protein purification construct may be employed in methods for expression of the product peptide in microbial and higher organism and for ligand immobilized affinity purification of the product peptide.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.