Calibration of a whole blood sample analyzer
US6127184A · kind A · utility
Assignees
Inventor
Key dates
| Filing date | Feb 10, 1999 |
| Grant date | Oct 3, 2000 |
| Priority date | — |
| Expiry date | Feb 10, 2019 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/115831
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Formed constituents of a quiescent anticoagulated whole blood or other biologic fluid sample are optically analyzed by an optical scanning instrument. The sample is contained in a sample chamber that has a varying through plane thickness. The thickness of any fields of view in the blood sample which contain plasma lacunae can be calculated by the instrument as a function of signal emission strength emanating from the colored plasma in the lacunae. The signal emissions can be the result of sample fluorescence or can be the result of signal density emanating from the sample. Particle volumes can be measured as a function of signal emission suppression which is caused by formed particles in the blood sample. The scanning instrument is calibrated by means of the inclusion of a calibration area associated with the chamber which calibration area includes a portion which receives a known depth of colored plasma from the blood sample, and which calibration area also includes a colorant-emission-suppressing feature which feature has a known volume. The scanning instrument scans the known depth portion of the calibration area to determine what degree of signal emission strength correlates to…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.