Apparatus and methods for active biological sample preparation
US6129828A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Sep 6, 1996 |
| Grant date | Oct 10, 2000 |
| Priority date | — |
| Expiry date | Sep 6, 2016 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01L2400/0644
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
Systems and methods for the electronic sample preparation of biological materials utilize the differential charge-to-mass ratio and/or the differential affinity of sample constituents to separation materials for sample preparation. An integrated system is provided for performing some or all of the processes of: receipt of biological materials, cell selection, sample purification, sample concentration, buffer exchange, complexity reduction and/or diagnosis and analysis. In one embodiment, one or more sample chambers adapted to receive a buffer solution are formed adjacent to a spacer region which may include a trap or other affinity material, electrophoretic motion of the materials to be prepared being effected through operation of electrodes. In another aspect of this invention, a transporter or dipstick serves to collect and permit transport of materials, such as nucleic acids, most preferably DNA and/or RNA. In one embodiment, a membrane or trap is held in a frame which is adapted to mate with a channel formed in the spacer region. In another aspect of this invention, an electrophoretic system for biological sample preparation is operated in a manner so as to utilize the differen…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.