Patent · US Expired

Method of Increasing activation on proliferation of T cells using antibody-microbead conjugates

US6129916A · kind A · utility

38Cited by
0References
5Claims
0Family size

Assignee

Inventor

Key dates

Filing dateNov 25, 1992
Grant dateOct 10, 2000
Priority date
Expiry dateNov 25, 2012

Classification

  • Technology area (CPC Y)Emerging Cross-Sectional Technologies
  • CPC primaryY10S530/866
  • WIPO fieldPharmaceuticals
  • WIPO sectorChemistry

Abstract

Disclosed are conjugates including a polymer backbone or microbead and binding molecules, such as Fv, Fab, or F(ab').sub.2 fragments of monoclonal antibodies or whole antibodies that are bound through their Fc carbohydrate moieties or have their Fc portion modified so that they cannot effect ADCC or complement-mediated cytolysis, and that are specific for a T cell surface antigen, such as CD3, TCR, CD4, CD8, or CD28 on T cells. The polymer or microbead is preferably made of cross-linked dextran, ficoll, latex, or agarose. The microbeads are preferably of 1 to 10 .mu.m in size, so that they can be suspended in in vivo fluids. These conjugates can be used to induce proliferation of T cells and immune stimulation, and to increase the antibody response against an administered antigen.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.