Chimaeric gene coding for a transit peptide and a heterologous polypeptide

US6130366A · kind A · utility

Assignees

• Plant Genetic Systems N.V. · BE
• BAYER HEALTHCARE LLC · DE

Inventors

• Luis Rafael Herrera-Estrella · Evergem, BE
• Guidi Van Den Broeck · Evergem, BE
• Marc Van Montagu · Brussels, BE
• Peter Schreier · Cologne, DE
• Josef Schell · Cologne, DE
• Hans J. Bohnert · Tucson, US
• Anthony R. Cashomore · New Rochelle, US
• Michael Paul Timko · Charlottesville, US
• Albert P. Kausch · Stonington, US

Key dates

• Filing date: Dec 3, 1997
• Grant date: Oct 10, 2000
• Expiry date: Dec 3, 2017

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/8221
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Chimaeric DNA sequence which encodes: 1) a transmit peptide of a cytoplasmic precursor of a chloroplast protein or polypeptide of a plant and 2) a protein or polypeptide that is heterologous to the transit peptide. The chimaeric DNA sequence can be used as a vector for transforming a plant cell so that a chimaeric precursor of the heterologous protein or polypeptide is produced in the cytoplasm of the cell and the chimaeric precursor then transports the heterologous protein or polypeptide in vivo into a chloroplast of the cell.