Murine leukemia virus vectors
US6132731A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Oct 8, 1997 |
| Grant date | Oct 17, 2000 |
| Priority date | — |
| Expiry date | Oct 8, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2810/855
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A proteinaceous particle comprises a capsid enveloped by ecotropic Murine Leukemia virus envelope proteins characterized in that a heterologous peptide which binds to a non-murine cell is inserted in, entirely replaces, or replaces a portion of the native Ser-Gly-Gly-Ser-Ser-Pro-Gly of the VRA region of said envelope proteins. A method for preparing a plurality of such proteinaceous particles comprises expressing within a host cell (i) self-assembling capsid proteins, (ii) Murine Leukemia virus envelope proteins, said ENV proteins modified as defined, and optionally, (iii) packageable RNA, and then culturing the host cells and harvesting the resultant budded particles.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.