Luciferases
US6132983A · kind A · utility
31Cited by
0References
32Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Nov 20, 1996 |
| Grant date | Oct 17, 2000 |
| Priority date | — |
| Expiry date | Nov 20, 2016 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y113/12007
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Proteins are provided having luciferase activity with greater heat stability than wildtype luciferases by replacing the glutamate equivalent to that at position 354 of Photinus pyralis luciferase or 356 of Luciola luciferases with an alternative amino acid, particularly lysine. DNA, vectors and cells that encode for and express the proteins are also provided as are test kits and reagents for carrying out luminescence assays using the proteins of the invention. Preferred proteins have a second replaced amino acid at a position equivalent to position 215 of Photinus pyralis luciferase or 217 of Luciola luciferases.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.