Tissue crosslinking for bioprostheses using activated difunctional or polyfunctional acids
US6132986A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 23, 1999 |
| Grant date | Oct 17, 2000 |
| Priority date | — |
| Expiry date | Apr 23, 2019 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61L2430/40
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The disclosed invention relates to the process of fixating tissue to obtain tissue for bioprosthetic applications. The fixating process comprises exposing the tissue to a fluid comprising activated difunctional or polyfunctional acids that can react with amino groups in tissue under mild conditions. Activating moieties include disuccinimidyl moieties, n-hydroxy disuccinimidyl moieties, sulfo-disuccinimidyl moieties, and mixtures thereof. The acid is preferably aliphatic difunctional or polyfunctional acids, more preferably a naturally occurring aliphatic difunctional or polyfunctional acids. Even more preferred is an acid consisting of glutaric, suberic acid, sebacic acid, tartaric acid, or mixtures thereof. The activated difunctional or polyfunctional acids are esters comprising the difunctional or polyfunctional acids and the activating moieties.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.