Enzymatic synthesis of chiral amines using -2-amino propane as amine donor

US6133018A · kind A · utility

Assignee

• Celgro · US

Inventors

• Wei Wu · Northville, US
• Mohit Bhatia · Manalapan, US
• Craig Matthew Lewis · Melbourne, AU
• Wei Lang · Edison, US
• Alice L. Wang · Green Brook, US
• George W. Matcham · Bound Brook, US

Key dates

• Filing date: Mar 10, 1999
• Grant date: Oct 17, 2000
• Expiry date: Mar 10, 2019

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P41/006
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

2-Aminopropane is used as the amine donor in the stereoselective synthesis of a chiral amine from a ketone with a transaminase. In a typical embodiment, (S)-1-methoxy-2-aminopropane is prepared by bringing methoxyacetone into contact with a transaminase in the presence of 2-aminopropane as an amine donor until a substantial amount of methoxyacetone is converted to (S)-1-methoxy-2-aminopropane and 2-aminopropane is converted to acetone. In a second embodiment, L-alanine is prepared by bringing pyruvic acid into contact with a transaminase in the presence of 2-aminopropane as an amine donor.