Transverse relaxation-optimized spectroscopy (=TROSY)
US6133736A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Oct 1, 1998 |
| Grant date | Oct 17, 2000 |
| Priority date | — |
| Expiry date | Oct 1, 2018 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61K49/06
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A method for obtaining a nuclear magnetic resonance (NMR) correlation spectrum of heteronuclear spin systems, in particular comprising large molecules, especially biological macromolecules in solution, the spin system being subjected to a homogeneous magnetic field B.sub.0, being irradiated by a sequence of radio frequency (rf) pulses, is characterized in that the spin system comprises at least two kinds of spin 1/2 nuclei I and S being coupled to each other, whereby the sequence of rf pulses is chosen such that line broadening in the observed spectrum due to transverse relaxation (T.sub.2) is significantly reduced because of cross correlation between dipole--dipole (DD) coupling of the spins and chemical shift anisotropy (CSA), giving rise to different relaxation rates of the individual multiplet components of the spin system and chosen such that the relaxation effects of the two different mechanisms cancel each other out to a large degree. Thus, even very large biological macromolecules can be measured.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.