Patent · US Expired

Method of cryopreserving hepatocytes

US6136525A · kind A · utility

2Cited by

0References

1Claims

0Family size

Assignee

Circe Biomedical, Inc. · US

Inventors

Claudy Jean Paul Mullon · Framingham, US
Shawn P. Cain · Chelmsford, US
Timothy Jon Perlman · Arlington, US
Hugo O. Jauregui · Providence, US
Sharda Naik · Providence, US
Henry A. Santangini · Providence, US
Donna M. Trenkler · Greene, US

Key dates

Filing date	Aug 2, 1999
Grant date	Oct 24, 2000
Priority date	—
Expiry date	Aug 2, 2019

Classification

Technology area (CPC A)Human Necessities
CPC primaryA61L27/3895
WIPO fieldBasic materials chemistry
WIPO sectorChemistry

Abstract

An artificial liver support system is described herein which comprises cryopreserved hepatocytes having an initial viability of 80-99%. Further disclosed are hepatocytes cryopreserved by dispensing hepatocytes into freezing containers, freezing the containers from between minus 50 to minus 90 degrees Celsius, storing the containers in liquid or vapor nitrogen, thawing the cryopreserved hepatocytes when ready for use and removing residual cryoprotectant media.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.