Patent · US Expired

High-affinity salicylic acid-binding protein and methods of use

US6136552A · kind A · utility

3Cited by

4References

7Claims

0Family size

Assignee

Rutgers, the State University of New Jersey · US

Inventors

Daniel F. Klessig · Bound Brook, US
He Du · Sunnyvale, US

Key dates

Filing date	Oct 23, 1997
Grant date	Oct 24, 2000
Priority date	—
Expiry date	Oct 23, 2017

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC07K14/415
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

A high-affinity salicylic acid-binding protein (SABP2) derivable from tobacco and Arabidopsis is disclosed. The tobacco protein has a molecular weight of approximately 25 kDa and reversibly binds SA with an apparent K.sub.d of approximately 90 nM and a B.sub.max of 10 fmol/mg protein. The SABP2 of the invention may be used to identify analogues of SA. Analogues so identified may be used in plants to augment disease-resistance response pathways or other SA-sensitive processes in which SA plays a role. Possible examples include flowering and alternative respiration. The SABP2 of the invention may also be used to identify and clone a gene or cDNA that encodes it, which then may be used to generate transgenic plants having altered SABP2 levels.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.