Purification method and apparatus
US6136555A · kind A · utility
26Cited by
5References
29Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Jan 4, 1996 |
| Grant date | Oct 24, 2000 |
| Priority date | — |
| Expiry date | Jan 4, 2016 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC40B60/14
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method is provided for purifying from cells a target compound such as nucleic acid. The method comprises the following steps: 1) lysing a cell suspension to form a cell lysate containing the target compound; 2) applying the cell lysate to a filter to remove unwanted cells and cell debris; 3) contacting the filtered lysate with a solid phase matrix under conditions to bind the nucleic acid to the matrix; 4) separating the resultant filtered lysate from the matrix; and 5) eluting the target compound from the matrix. Apparatus is also provided. Complex purification procedures such as centrifugation are avoided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.