Reverse transcriptase of moloney murine leukemia virus with RNA polymerase activity
US6136582A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jan 20, 1998 |
| Grant date | Oct 24, 2000 |
| Priority date | — |
| Expiry date | Jan 20, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/1276
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention provides a polypeptide capable of polymerizing i) deoxyribonucleotides; ii) ribonucleotides; or iii) one or more deoxyribonucleotides and one or more ribonucleotides. This invention further provides a purified Moloney murine leukemia virus reverse transcriptase, wherein an amino acid corresponding to position 155 of a wild type Moloney murine leukemia virus reverse transcriptase is a valine. This invention also provides a method of polymerizing a nucleic acid molecule comprising one or more deoxyribonucleotides and one or more ribonucleotides comprising: contacting a polypeptide capable of polymerizing i) deoxyribonucleotides; ii) ribonucleotides; or iii) one or more deoxyribonucleotides and one or more ribonucleotides with deoxyribonucleotides and ribonucleotides under conditions permitting incorporation of deoxyribonucleotides and ribonucleotides into a nucleic acid molecule. This invention still further provides a method of converting a DNA polymerase into an RNA polymerase comprising: a) structurally modifying the DNA polymerase; b) contacting the structurally modified DNA polymerase of step (a) with deoxyribonucleotides and ribonucleotides under conditions permi…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.