Genes encoding denitrification reactions
US6136588A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jul 15, 1999 |
| Grant date | Oct 24, 2000 |
| Priority date | — |
| Expiry date | Jul 15, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/52
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention relates to the isolation of nucleic acid fragments from Pseudomonas sp. strain G-179 that encode periplasmic nitrate reductase and nitric oxide reductase enzymes. The enzymes are useful in denitrification reactions and for the identification of other denitrifying bacteria. In addition, this invention also relates to the construction of chimeric genes encoding all or a substantial portion of a bacterial nitric oxide reductase or a bacterial periplasmic nitrate reductase enzymes, in sense or antisense orientation, wherein the expression of the chimeric genes results in production of altered levels of the nitric oxide reductase or periplasmic nitrate reductase in a transformed host cell.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.