Homologous recombination for allogeneic donor cells

US6139835A · kind A · utility

Assignee

• Cell Genesys, Inc. · US

Inventors

• Raju Kucherlapati · Darien, US
• Beverly Koller · Carrboro, US
• Oliver Smithies · Durham, US
• Robert B. Dubridge · Belmont, US
• Gary Greenburg · Redwood City, US
• Daniel J. Capon · Hillsborough, US
• Steven R. Williams · Colorado Springs, US
• Mariona Lourdes Arbones De Rafael · Barcelona, ES

Key dates

• Filing date: May 18, 1995
• Grant date: Oct 31, 2000
• Expiry date: May 18, 2015

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A01K2267/0381
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Homologous recombination is employed to inactivate genes, particularly genes associated with MHC antigens. Particularly, the .beta..sub.2- microglobulin gene is inactivated for reducing or eliminating the expression of functional Class I MHC antigens. The resulting cells may be used as allogeneic donor cells. Methods for homologous recombination in non-transformed mammalian somatic cells are also described.