DNA sequencing by mass spectrometry via exonuclease degradation
US6140053A · kind A · utility
287Cited by
97References
44Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Sep 25, 1998 |
| Grant date | Oct 31, 2000 |
| Priority date | — |
| Expiry date | Sep 25, 2018 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N35/1067
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention provides fast and highly accurate mass spectrometer based processes for directly sequencing a target nucleic acid (or fragments generated from the target nucleic acid), which by means of protection, specificity of enzymatic activity, or immobilization, are unilaterally degraded in a stepwise manner via exonuclease digestion and the nucleotides, derivatives or truncated sequences detected by mass spectrometry.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.