SH3 protein, gene, chimeric cells, vectors and expression method for producing the novel protein, and uses

US6140074A · kind A · utility

Inventors

• Timothy J. O'Brien · St. Francis, US
• Yinxiang Wang · Beijing, CN

Key dates

• Filing date: Jun 9, 1997
• Grant date: Oct 31, 2000
• Expiry date: Jun 9, 2017

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N9/6424
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention is a protein having the amino acid sequence of Seq. I.D. No. 1 or an allelic variation retaining the biological activity of the protein having the amino acid sequence of Seq. I.D. No. 1, a DNA segment coding for a protein according to claim 1, preferably DNA segment according to claim 2 having the sequence of Seq. I.D. No. 2, or a substitution analog or allelic variation of Seq. I.D. No. 2, a chimeric cell comprising the DNA segment coding for a protein of Seq. I.D. No. 1, preferably a chimeric cell comprising the DNA segment of Seq. I.D. No. 2, a vector comprising a DNA segment coding for a protein having Seq. I.D. No. 1 operably linked to a promoter. The invention provides a preferred vector comprising the following components operably linked from 5' to 3': (a) a promoter; (b) a signal sequence; (c) 5' portion of a highly expressed gene endogenous to a selected host cell, (d) a linker sequence; all preceding the nucleotide sequence coding for TADG5 protein. The invention provides a protein production method which comprises expressing a DNA segment coding for a protein with the amino acid sequence of Seq. I.D. No. 1 in a chimeric host cell, preferably one which compr…