Method for producing phytase
US6140077A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jan 28, 1998 |
| Grant date | Oct 31, 2000 |
| Priority date | — |
| Expiry date | Jan 28, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/815
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for producing phytase using a Candida boidinii transformant, by which phytase is secreted to liquid medium without substantially increasing the number of the transformant cells is disclosed. In this method, cells of a Candida boidinii transformant which is transformed with a recombinant vector comprising a promoter inducible with methanol and a gene located downstream of said promoter and operably linked to said promoter, which gene encodes a polypeptide having phytase activity, are cultured in a liquid culture medium containing phosphoric acid or a phosphate compound in an amount effective of restricting proliferation of said cells of Candida boidinii transformant such that the amount of said cells of Candida boidinii transformant is not substantially increased, while adding methanol to the culture medium, so as to make said cells secrete said phytase.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.