Protein with phospholipase activity

US6140094A · kind A · utility

Assignee

• Rohm Co., Ltd. · JP

Inventors

• Fridolin Loffler · Bensheim, DE
• Gerald Jungschaffer · Zwingenberg, DE
• Quoc Nguyen Khanh · Reichelsheim, DE
• Erwin Schuster · Bechhofen, DE
• Bruno Sprossler · Roßdorf, DE
• Sabine Wolf · Otzberg, DE

Key dates

• Filing date: Sep 8, 1998
• Grant date: Oct 31, 2000
• Expiry date: Sep 8, 2018

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S435/917
• WIPO field: Food chemistry
• WIPO sector: Chemistry

Abstract

This invention relates to a protein having phospholipase activity, which is characterised in that it has the mature sequence of Aspergillus lysophospholipase or a sequence derived therefrom and that it may be cleaved at at least one site, wherein, in the event of cleavage, the restriction fragments are optionally either linked by means of at least one bond cleavable under reducing conditions or at least one of the unlinked restriction fragments has phospholipase activity, and to a process for the production of this protein by fermenting a suitably transformed lysophospholipase-producing host organism in a suitable culture medium and isolating the protein having phospholipase activity from the cell-free culture filtrate, wherein fermentation is performed in the acidic to slightly alkaline range.