Method and apparatus for identifying classifying or quantifying DNA sequences in a sample without sequencing
US6141657A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Oct 1, 1997 |
| Grant date | Oct 31, 2000 |
| Priority date | — |
| Expiry date | Oct 1, 2017 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S707/99936
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention provides methods by which biologically derived DNA sequences in a mixed sample or in an arrayed single sequence clone can be determined and classified without sequencing. The methods make use of information on the presence of carefully chosen target subsequences, typically of length from 4 to 8 base pairs, and preferably the length between target subsequences in a sample DNA sequence together with DNA sequence databases containing lists of sequences likely to be present in the sample to determine a sample sequence. The preferred method uses restriction endonucleases to recognize target subsequences and cut the sample sequence. Then carefully chosen recognition moieties are ligated to the cut fragments, the fragments amplified, and the experimental observation made. Polymerase chain reaction (PCR) is the preferred method of amplification. Another embodiment of the invention uses information on the presence or absence of carefully chosen target subsequences in a single sequence clone together with DNA sequence databases to determine the clone sequence. Computer implemented methods are provided to analyze the experimental results and to determine the sample sequences in…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.