Method for the affinity-chromatographic purification of factor VIII

US6143179A · kind A · utility

Assignee

• Kerckhoff-Klinik Gesellschaft mit beschrankter Haftung · DE

Inventors

• Gert Muller-Berghaus · Ober-Mörlen, DE
• Bernd Pötzsch · Bad Salzuflen, DE
• Horst Schwinn · Mainz, DE

Key dates

• Filing date: Dec 22, 1998
• Grant date: Nov 7, 2000
• Expiry date: Dec 22, 2018

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K14/755
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention concerns a process for the purification of factor VIII by affinity chromatography characterized in that a biological fluid containing factor VIII is contacted with immobilized cellular von Willebrand factor or a derivative thereof under such conditions that factor VIII is adsorbed to the immobilized von Willebrand factor or derivative thereof, impurities are separated and adsorbed factor VIII is eluted.