Method for the preparation of N-glycolyl neuraminic acid from Cucumaria echinata
US6143533A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 13, 2000 |
| Grant date | Nov 7, 2000 |
| Priority date | — |
| Expiry date | Mar 13, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P7/58
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed is an efficient and economical method for the preparation of N-glycolyl neuraminic acid in a high purity from an inexpensive abundant source material. The method comprises dispersing body tissues of an echinodermatous marine animal Cucumaria echinata in an aqueous medium, preferably, using a dry powder of the tissues prepared in advance, in which the tissues are proteolytically decomposed to isolate N-glycolyl neuraminic acid in the form of an aqueous solution containing polypeptides as a by-product, followed by separation of N-glycolyl neuraminic acid from the aqueous solution by removing the polypeptides and purification of the compound in a process utilizing an ion-exchange treatments.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.