Carbon-based process for selection of transgenic plant cells
US6143562A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 12, 1998 |
| Grant date | Nov 7, 2000 |
| Priority date | — |
| Expiry date | May 12, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/8209
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A carbon-based process for the selection of heterotrophically cultured plant cells is contemplated as are plants transformed using that process and a kit useful for effecting such a transformation. Plant cells are transformed with a heterologous DNA segment that contains two expression cassettes. The first cassette contains a gene that encodes a heterologous enzyme that on expression converts a growth-limiting (encrypted) carbon source that does not support growth and proliferation of non-transformed plant cells into a carbon source that supports growth and proliferation of transformed plant cells. The second cassette contains a second gene to be expressed in the transformed plant cells. A mixture transformed and non-transformed plant cells is cultured under heterotrophic culture conditions with the growth-limiting carbon source as the only carbon source. Inasmuch as only the transformed cells express the heterologous enzyme that converts the carbon source present into a carbon source that is useful to support growth and proliferation, only transformed cells grow and proliferate, and those cells are selected.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.