Modified pseudomonas oleovorans phaC1 nucleic acids encoding bispecific polyhydroxyalkanoate polymerase
US6143952A · kind A · utility
15Cited by
15References
24Claims
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Key dates
| Filing date | Mar 31, 1998 |
| Grant date | Nov 7, 2000 |
| Priority date | — |
| Expiry date | Mar 31, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P7/625
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A genetically engineered Pseudomonas oleovorans phaC1 polyhydroxyalkanoate (PHA) polymerase having tailored substrate specificity is provided. The modified PHA polymerase is preferably a "bispecific" PHA polymerase capable of copolymerizing a short chain length monomer and a medium chain length monomer is provided. Methods for making the modified PHA polymerase and for making nucleic acids encoding the modified PHA polymerase are also disclosed, as are methods of producing PHA using the modified PHA polymerase. The invention further includes methods to assay for altered substrate specificity.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.