High-throughput screening assays utilizing metal-chelate capture
US6146842A · kind A · utility
31Cited by
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19Claims
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Key dates
| Filing date | Sep 17, 1999 |
| Grant date | Nov 14, 2000 |
| Priority date | — |
| Expiry date | Sep 17, 2019 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/91
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A high throughput enzyme screen has been developed which relies on metal chelate interaction for capture of the product of the enzymatic reaction. In the present assay system, a detectable moiety is attached to a substrate having a chelating capturable moiety, which can be captured by an immobilized metal. Detection is effected due to the presence of a detectable label on the reaction product immobilized on the solid phase. Only signal associated with tagged protein bound to the solid phase is detected. The present assay can reliably measure enzyme activity, and has high reproducibility, which benefits high throughput screening.
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