Method and system for cultivating macrophages
US6146890A · kind A · utility
Inventor
Key dates
| Filing date | Mar 21, 1997 |
| Grant date | Nov 14, 2000 |
| Priority date | — |
| Expiry date | Mar 21, 2017 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61K2035/124
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A method of cultivating macrophages from blood, including collecting a quantity of blood, fractionating the quantity of blood into a plasma fraction, a white blood cell fraction generally including monocytes, and a red blood cell fraction, segregating the white blood cell fraction from the plasma fraction, while allowing a portion of the red blood cell fraction to remain mixed with the white blood cell fraction, the portion of the red blood cell fraction being less than the white blood cell fraction, and inducing differentiation of the monocytes into macrophages and lysing at least part of the portion of the red blood cell fraction mixed with the white blood cell fraction by causing an osmotic shock to the white blood cell fraction and the red blood cell fraction
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.