Nucleic acid detection probes having non-FRET fluorescence quenching and kits and assays including such probes

US6150097A · kind A · utility

Assignee

• The Public Health Research Institute Of the City of New York, Inc. · US

Inventors

• Sanjay Tyagi · New York, US
• Fred R. Kramer · Yonkers, US

Key dates

• Filing date: Dec 12, 1997
• Grant date: Nov 21, 2000
• Expiry date: Dec 12, 2017

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S436/80
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Nucleic acid hybridization probes having a first conformation when not interacting with a target and a second conformation when interacting with a target, and having the ability to bring a label pair into touching contact in one conformation but not the other, are labeled with a non-FRET pair of chromophores and generate a fluorescent or absorbance signal. Kits may include such probes, and assays, including multiplex assays, may utilize such probes.