Polyethylenimine-based biomolecule arrays
US6150103A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jul 21, 1998 |
| Grant date | Nov 21, 2000 |
| Priority date | — |
| Expiry date | Jul 21, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC40B60/14
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
An array of biomolecules is formed from a solid substrate comprising a surface, where said surface is at least partially covered with a layer of poly(ethylenimine) (PEI), and the layer is divided among a plurality of discrete first regions abutted and surrounded by a contiguous second region. The first regions are defined by the presence of a biomolecule and PEI. The second region is defined by the presence of PEI and the substantial absence of the biomolecule. The array may be prepared by a process including the steps of providing a solid substrate having a surface, wherein a layer of poly(ethylenimine) (PEI) covers at least a portion of the surface. The layer contains a plurality of discrete first regions abutted and surrounded by a contiguous second region. The process includes the step of depositing a biomolecule into the first regions while maintaining the second region substantially free of the biomolecule.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.