Patent · US Expired

DNA strands useful for the synthesis of xanthophylls and the process for producing the xanthophylls

US6150130A · kind A · utility

15Cited by
0References
12Claims
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Key dates

Filing dateJun 18, 1999
Grant dateNov 21, 2000
Priority date
Expiry dateJun 18, 2019

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12P23/00
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

Disclosed are the following DNA strands relating to the synthesis of keto group-containing xanthophylls such as astaxanthin and the like, and the techniques relating to the production of xanthophylls by genetic engineering: A DNA strand having a nucleotide sequence which encodes a polypeptide having an enzyme activity for converting a methylene group at the 4-position of a .beta.-ionone ring into a keto group. A DNA strand having a nucleotide sequence which encodes a polypeptide having an enzyme activity for converting a methylene group at the 4-position of a 3-hydroxy-.beta.-ionone ring into a keto group. A DNA strand having a nucleotide sequence which encodes a polypeptide having an enzyme activity for adding a hydroxyl group to the 3-carbon of a 4-keto-.beta.-ionone ring. It is possible to produce a variety of xanthophylls such as canthaxanthin, astaxanthin and the like by introducing the DNA strands into an appropriate microorganism such as Escherichia coli and the like.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.