Intron-mediated recombinant techniques and reagents
US6150141A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Mar 11, 1997 |
| Grant date | Nov 21, 2000 |
| Priority date | — |
| Expiry date | Mar 11, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2840/445
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention makes available methods and reagents for novel manipulation of nucleic acids. As described herein, the present invention makes use of the ability of intronic sequences, such as derived from group I, group II, or nuclear pre-mRNA introns, to mediate specific cleavage and ligation of discontinuous nucleic acid molecules. For example, novel genes and gene products can be generated by admixing nucleic acid constructs which comprise exon nucleic acid sequences flanked by intron sequences that can direct trans-splicing of the exon sequences to each other. The flanking intronic sequences can, by intermolecular complementation, form a reactive complex which promotes the transesterification reactions necessary to cause the ligation of discontinuous nucleic acid sequences to one another, and thereby generate a recombinant gene comprising the ligated exons.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.