Chondrocyte media formulations and culture procedures
US6150163A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jan 13, 1999 |
| Grant date | Nov 21, 2000 |
| Priority date | — |
| Expiry date | Jan 13, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2501/58
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
One object of the present invention is based upon the development and use of a serum-free defined cell culture medium comprising a supplement mixture, a component mixture, a vitamin mixture, an inorganic salt mixture and amino acid mixture that avoids the problems inherent in the use of serum. In particular, the defined medium is useful in culturing fibroblasts, especially chondrocytes. Another object of the present invention is to claim a method of enhancing the differentiation of chondrocytes and enhancing the synthesis of a cartilage specific matrix using tumor growth factor beta (TGF-.beta.). Another object of the present invention is to claim a method of enhancing the differentiation of chondrocytes using the combination of TGF-.beta.and IGF.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.