Expression of the heterologous genes according to a targeted expression profile
US6150169A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jan 25, 1996 |
| Grant date | Nov 21, 2000 |
| Priority date | — |
| Expiry date | Jan 25, 2016 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2840/44
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention relates to DNA constructs for inserting heterologous gene sequences into a host genome so as to obtain expression of the heterologous gene, to methods of inserting heterologous gene sequences into a host genome, and to organisms carrying modified host genomes. Specifically, the DNA constructs of this invention contain an expression unit of an internal ribosome binding site (IRES) coupled to a heterologous gene sequence. This expression unit is flanked at the 5' and 3' ends by DNA sequences that enable homologous recombination or integration of the construct with the DNA of a targeted host to obtain expression of the heterologous gene in the host.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.