Magnetic nanoparticles coupled to annexine, and utilization thereof
US6150181A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jun 11, 1998 |
| Grant date | Nov 21, 2000 |
| Priority date | — |
| Expiry date | Jun 11, 2018 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T428/2991
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention relates to a process for producing ferrofluid-forming magnetic particles substituted by DMSA (FFSH) that can be covalently coupled, directly or through a difunctional reactant, to an effector, which process comprises combining DMSA with ferrofluid-forming magnetic particles to form FFSS particles having disulfide bonds, and reducing the disulfide bonds of the FFSS at basic pH to form FFSH. The invention includes a composition comprising such FFSH magnetic particles. The invention also relates to a method in which FFSH magnetic particles are incubated with an effector comprising at least one functional group which forms an S--S, C--S, C--C, or C--N bond with DMSA, so that a covalent effector-FFSH complex is formed. The invention further includes a method for separating and/or distinguishing between molecules or cells having a ligand that forms an affinity complex with an effector-FFSH particle of the invention, and those molecules or cells that do not have such a ligand.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.