Borrelia burgdorferi outer membrane proteins

US6153194A · kind A · utility

Assignee

• The Regents of the University of California · US

Inventors

• Jonathan T. Skare · College Station, US
• Ellen S. Shang · Los Angeles, US
• Cheryl I. Champion · Culver City, US
• David R. Blanco · San Fernando, US
• James N. Miller · San Fernando, US
• Michael A. Lovett · Los Angeles, US
• Tajib Mirzabekov · Palo Alto, US
• Bruce L. Kagan · Santa Monica, US
• Paul Tempst · New York, US
• Denise Foley · Orange, US

Key dates

• Filing date: Oct 29, 1998
• Grant date: Nov 28, 2000
• Expiry date: Oct 29, 2018

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02A50/30
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The present invention presents three B. burgdorferi membrane proteins: Oms28, Oms45, and Oms66, each of about 28, 45, and 66 kDa respectively; and with average single channel conductances of about 0.6, 0.22, and 9.7 nS, respectively. Also disclosed are the methods for purifying these proteins from B. burgdorferi, methods for producing antibodies to these proteins, and the resulting antibodies. These proteins and their immunogenic fragments, and antibodies capable of binding to them are useful for inducing an immune response to pathogenic B. burgdorferi as well as providing a diagnostic target for Lyme disease. Further disclosed are the nucleotide and amino acid sequences, the cloning of the genes encoding the proteins and their recombinant proteins, and methods for obtaining the foregoing. Other B. burgdorferi outer membrane spanning proteins (Oms) obtainable by the isolation and purification methods of the present invention.