High throughput screening assays for nucleic acid ligase modulators
US6153384A · kind A · utility
4Cited by
1References
47Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Feb 19, 1998 |
| Grant date | Nov 28, 2000 |
| Priority date | — |
| Expiry date | Feb 19, 2018 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2500/02
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Screening assays for identifying ligase activity modulators are provided, in both solid phase and liquid phase formats. Solid phase formats detect ligase activity by ligating a labelled nucleic acid to a capture nucleic acid in the presence of the ligase modulator and detection of the labelled nucleic acid. Liquid phase assays detect ligation-dependent changes in interactive labels between nucleic acids such as proximity quenching of fluorescent labels. Compositions, apparatus and integrated systems for assays are also provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.