Method for producing amide compounds using a nitrile hydratase from a thermophilic bacillus
US6153415A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Feb 10, 1999 |
| Grant date | Nov 28, 2000 |
| Priority date | — |
| Expiry date | Feb 10, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12R2001/07
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A process for the bioconversion of a nitrile to its corresponding amide product, particularly acrylonitrile to acrylamide which is used for forming polymers. The process uses a thermophilic bacterium having a nitrile hydratase activity that is constitutively expressed, activated by cobalt ions, stable at 60.degree. C., and is most active between 20.degree. C. to 70.degree. C. with optimum activity at 55.degree. C. Alternatively, the process uses the enzyme extracted from the thermophilic bacterium to convert a nitrile to its amide product. The genes encoding nitrile hydratase and amidase are described in which the former is useful for the conversion of an nitrile to its amide and the later is useful for the conversion of an amide to its acid.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.