Method for quantitative determination of 1,5-anhydroglucitol
US6153419A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Oct 16, 1997 |
| Grant date | Nov 28, 2000 |
| Priority date | — |
| Expiry date | Oct 16, 2017 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/872
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a method for quantitative determination of 1,5-anhydroglucitol in a sample, which comprises mixing the sample and an enzyme having activity that is inhibited by 1,5-anhydroglucitol in a concentration-dependent manner, and measuring the activity of the enzyme; and a reagent for quantitative determination of 1,5-anhydroglucitol which comprises an enzyme having activity that is inhibited by 1,5-anhydroglucitol in a concentration-dependent manner, a substrate for the enzyme, and a reagent for quantitative determination of a product formed by the enzyme activity. The present invention also relates to novel trehalase having a Ki value of 0.33 mM or less for 1,5-anhydroglucitol; and a process for producing novel trehalase having the above-mentioned physicochemical properties, which comprises culturing in a medium a microorganism belonging to the genus Nocardia and capable of producing the trehalase, and recovering the trehalase from the culture.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.