Modified-affinity streptavidin
US6156493A · kind A · utility
9Cited by
9References
13Claims
0Family size
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Key dates
| Filing date | Sep 30, 1997 |
| Grant date | Dec 5, 2000 |
| Priority date | — |
| Expiry date | Sep 30, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K14/36
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Streptavidin tetramers have at least one monomer containing an amino acid modification that produces a reduced binding affinity for biotin, a modified off-rate, a modified on-rate, or an altered binding enthalpy. Polynucleotides encoding the modified monomers are also provided. The modified streptavidin and chimeric streptavidin molecules are useful in methods of bioseparations and cell sorting, imaging, drug delivery, and diagnostics.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.