Recombinase-mediated generation of adenoviral vectors
US6156497A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Apr 13, 1998 |
| Grant date | Dec 5, 2000 |
| Priority date | — |
| Expiry date | Apr 13, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2830/38
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for generating adenoviral vectors from polynucleotides such as plasmids wherein there occurs recombinase-mediated transfer of an adenoviral ITR and terminal proteins bound to the ITR to a plasmid, thus enabling the plasmid to replicate as an adenoviral vector. Such method enables the rapid generation of adenoviral vectors at high titers from plasmids without the use of selectable markers and screening procedures. Such method enables the rapid generation of adenoviral vectors devoid of adenovirus backbone genes. The method also may be employed to generate hybrid adenoviral-retroviral vectors that convert transduced cells into producer cells that produce retroviral vectors to effect high level, permanent genetic modification of cells in vivo.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.